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GATE Parents Group

serving the families of the San Mateo Union High School District

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Summer Enrichment: Biotechnology Boot Camp

Description
DNA extraction, polymerase chain reactions, gel electrophoresis, laboratory skills - do it all! See Class Sessions below for details.

Please note: Class includes lunch period, bring your own lunch from Monday through Thursday. Lunch is included on Friday. Registration includes cost of all lab supplies and evaluation lunch.
Instructor
Jimmy Ikeda
Fee
$485.00, Early Registration: $400.00
Maximum Students
30
Registration Dates
All Student Registration Period
4/15/2019 through 6/10/2019
Enrollment Period
4/15/2019 through 6/10/2019
Fee Dates
Discounted Fee Registration Period
4/15/2019 through 4/28/2019
Regular Fee Registration Period
4/29/2019 through 6/10/2019
Class Sessions
TitleDayDateTimeLocationDescription
DayMonday6/17/20199:00 am to 3:00 pmSan Mateo High School Micropipetting skills/ Pipetting Secret Code DNA Spooling from Salmon Sperm DNA and strawberries Solution and Media Prep (LB Agar and LB Broth) Electrophoresis basics and gel electrophoresis Poster Introduction and preparation – choice of drug research or application of Biotechnology
DayTuesday6/18/20199:00 am to 3:00 pmSan Mateo High SchoolCheek Cell Sterile technique introduction Pour LB plates for bacteria cell culture Natural Products Drug Discovery (NPDD) Bioassays Introduction Plant protein extract DNA extraction introduction Cheek cell DNA extraction Polymerase Chain Reaction – Searching for the ALU DNA insert introduction Thin layer chromatography of plant extracts Agarose prep for Alu PCR gel electrophoresis Poster preparation – choice of drug research or application of Biotechnology
DayWednesday6/19/20199:00 am to 3:00 pmSan Mateo High SchoolGel Electrophoresis of Alu DNA and research NPDD extract preparation Bacterial transformation of pGLO Plate bacteria and incubate Results of ALU PCR Wolbachia PCR introduction Insect search and DNA removal Wolbachia PCR Poster preparation – choice of drug research or application of Biotechnology
DayThursday6/20/20199:00 am to 3:00 pmSan Mateo High SchoolWolbachia PCR gel electrophoresis Enzyme-Linked Immunosorbant Assay (ELISA) background ELISA of animal proteins Protein extraction from meat samples and dilutions Polyacrylamide Gel Electrophoresis (PAGE) of protein samples Wolabchia PCR results Guest Speaker Poster preparation – choice of drug research or application of Biotechnology
DayFriday6/21/20199:00 am to 3:00 pmSan Mateo High School Gel electrophoresis of protein results pGLO Transformation results Summarize results, Lab notebooks Lab cleanup, Evaluation Lunch – Parents, please join us! Poster presentation on drug research, company, career path